Anti-NR1H4 Antibody

NR1H4 encodes the Farnesoid X receptor (FXR), a ligand-activated nuclear transcription factor that senses intracellular bile acids and coordinates expression of downstream genes controlling bile acid, glucose, and lipid homeostasis. The polyclonal antibody raised against this target is produced in rabbits and purified as an IgG immunoglobulin, supplied unconjugated by Antibodies.com in a 200 µl volume for use in Western blot workflows.

The reagent detects endogenous NR1H4 in human, mouse, and rat samples, enabling translational studies of hepatic and intestinal FXR signaling relevant to cholestasis, non-alcoholic fatty liver disease, and broader metabolic regulation. Its unconjugated format lets researchers pair the primary antibody with HRP, fluorophore, or biotin-conjugated secondary reagents suited to their specific membrane-based detection chemistry.

Typical Western blot applications include probing whole-cell or tissue lysates to visualize FXR protein, expected at approximately 50-55 kDa depending on isoform and post-translational modification. The polyclonal composition broadens epitope coverage across the receptor, which can be advantageous when characterizing splice variants or modified forms of FXR in primary hepatocytes, enterocytes, or relevant cell line models.