Anti-NQO1 Antibody

NQO1 (NAD(P)H:quinone dehydrogenase 1), also referred to as DT-diaphorase, is a cytosolic flavoenzyme that catalyzes the obligatory two-electron reduction of quinones to hydroquinones, contributing to cellular defense against oxidative stress and electrophilic toxicity. This unconjugated rabbit polyclonal primary antibody from Antibodies.com was raised against the human NQO1 protein and is supplied as 100 µl of antiserum for use in western blot and ELISA applications. The reagent specifically detects the human target, making it suitable for research into xenobiotic metabolism, redox homeostasis, and the Nrf2 antioxidant response pathway, where NQO1 is a well-characterized downstream effector. Investigators commonly deploy anti-NQO1 antibodies to study chemoprotection, tumor biology, and cellular responses to quinone-based chemotherapeutics. Aliquot the vial upon arrival and store refrigerated in the dark to preserve antibody reactivity across multiple experiments; the polyclonal format recognizes multiple epitopes on the approximately 31 kDa NQO1 protein, an advantage when probing denatured whole-cell lysates on western blot membranes.