Anti-NQO1 Antibody

Raised in rabbit and purified by affinity chromatography against the immunogen, this polyclonal reagent from Antibodies.com targets NAD(P)H:quinone dehydrogenase 1 (NQO1) and is supplied at 1.0 mg/mL in a 50 µL volume. The host species is rabbit, the clonality is polyclonal, and the antibody is provided unconjugated, making it suitable for direct labeling or secondary-detection workflows. According to the supplier datasheet, it has been validated for Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) applications, and it detects the endogenous total NQO1 protein in human, mouse, and rat samples. The immunogen is a synthetic peptide derived from human NQO1, supporting broad cross-reactivity across these three species. The formulation is a phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide as a preservative, and 50% glycerol for cryoprotection, which is consistent with standard short- to long-term storage at −20 °C with minimal freeze–thaw cycling. The reagent is intended for research use only and is shipped on blue ice from the Antibodies.com distribution center in St. Louis, MO. This Antibodies.com polyclonal anti-NQO1 antibody is a reliable choice for laboratories studying the Nrf2/ARE signaling pathway, oxidative stress responses, quinone detoxification, and cancer biology where NQO1 expression is routinely assessed by immunoblotting and fluorescence-based imaging.