Anti-NPM1 Antibody

This unconjugated mouse monoclonal IgG1 antibody targets nucleophosmin (NPM1), a nucleolar phosphoprotein that participates in ribosomal RNA processing, centrosome duplication, and regulation of the ARF-p53 tumor suppressor pathway. The antibody is supplied as 100 µl in a vial and is validated for western blot and immunocytochemistry workflows, making it useful for examining nucleolar architecture and endogenous NPM1 expression in fixed cells and lysate preparations.

Reactivity has been confirmed with human, mouse, rat, and rabbit samples, so the reagent can be applied across comparative studies and multiple model systems. As a monoclonal reagent, it offers lot-to-lot consistency for reproducible detection of the target, and the unconjugated format gives researchers flexibility to pair it with a secondary antibody of their choice (HRP, fluorophore, or biotin conjugates) suited to their detection platform.

NPM1 is of particular clinical and research interest because nucleophosmin gene mutations and aberrant cytoplasmic localization are hallmarks of a molecular subtype of acute myeloid leukemia; reliable antibodies against this target support studies of nucleolar stress, ribosome biogenesis, and myeloid malignancy biology. From Antibodies.com, this primary antibody is intended for research use only.