Anti-NPM (phospho-T234) Antibody

This rabbit polyclonal reagent from Antibodies.com specifically recognizes nucleophosmin (NPM1) when phosphorylated at threonine 234, a post-translational mark linked to CDK2/cyclin E-driven G1/S transition and licensing of centrosome duplication. Each vial contains 100 µl of IgG formulated at 1 mg/ml in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2, supplied unconjugated for direct use in standard western blot and immunohistochemistry workflows.

Validated for reactivity against human, mouse, and rat samples, the antibody supports studies of nucleolar dynamics, ribosome biogenesis, and p53-pathway signaling. The phospho-T234 epitope is widely examined in proliferating tumor cells, where aberrant NPM phosphorylation has been linked to mitotic defects and genomic instability. The polyclonal format provides broader epitope coverage than a monoclonal reagent, which can improve detection across minor sequence variations between species and increase tolerance to partial protein degradation in fixed samples.

Store refrigerated upon arrival; aliquot and freeze at -20 °C for long-term use. Protect from light and avoid repeated freeze-thaw cycles to preserve phospho-specific binding activity. Each lot is quality-controlled by the supplier to confirm selective recognition of the phosphorylated epitope over the unmodified counterpart.