Anti-NPHS1 Antibody

Produced in rabbit as an unconjugated polyclonal reagent, this anti-nephrin antibody from Antibodies.com recognizes NPHS1, the transmembrane scaffolding protein that forms the structural core of the slit diaphragm between adjacent podocyte foot processes in the renal glomerular filtration barrier. Supplied at 100 µl, the antibody is validated for western blot and immunohistochemistry workflows on human and mouse samples, supporting studies of podocyte injury, glomerular barrier integrity, and disease models such as congenital nephrotic syndrome of the Finnish type and focal segmental glomerulosclerosis.

The polyclonal host species and unconjugated format give laboratories flexibility to pair the primary with their preferred HRP-, biotin-, or fluorophore-conjugated secondary detection system, whether the application calls for chromogenic visualization on fixed kidney sections or chemiluminescent detection of the ~180 kDa band in denatured lysates. Refrigerated storage between uses is recommended, along with aliquoting to limit repeated freeze-thaw cycles that can erode titre over the working shelf life. Intended for research use only, this reagent provides a reliable tool for tracking nephrin expression, distribution, and turnover in normal glomeruli and across experimental and disease states.