Anti-NOX5 Antibody

Rabbit polyclonal IgG antibody targeting NOX5, offered unconjugated by Antibodies.com. The reagent is supplied as a liquid and is raised in rabbit using a polyclonal approach.

Validated applications cited across sources include Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry (IHC), and ELISA. Reactivity is reported for human, mouse, and rat samples across different product references, with one source noting the antibody detects endogenous levels of total NOX5 protein. Recommended working dilutions include WB at 1:500–1:1,000 and 1:500–1:2,000, ICC/IF at 1:50–1:200, and ELISA at 1:40,000. One source describes the immunogen as a recombinant fusion protein containing a sequence corresponding to amino acids 1–220 of human NOX5 (NP_078781.3), another as a synthetic peptide derived from human NOX5 amino acids 677–726, and a third as a fusion protein corresponding to residues near the C-terminal of human NADPH oxidase EF-hand calcium binding domain 5.

Formulations referenced include Phosphate Buffered Saline at pH 7.3 with 50% glycerol and 0.01% Thiomersal; PBS at pH 7.3 with 0.05% sodium azide and 50% glycerol; and Phosphate Buffered Saline without Mg2+ and Ca2+ at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. Reported molecular weight values for the target are 75 kDa and 86 kDa across sources. Purification is described as affinity purification or antigen affinity chromatography. The product is shipped at 4°C; upon delivery, aliquoting and storage at -20°C is recommended, with freeze/thaw cycles to be avoided.