Anti-NOX4 Antibody

NOX4 (NADPH Oxidase 4) is a constitutively active member of the NADPH oxidase family that generates hydrogen peroxide as its primary reactive oxygen species output, distinguishing it from other NOX isoforms that depend on cytosolic subunit assembly for activation. This unconjugated rabbit polyclonal antibody from Antibodies.com targets NOX4 and is validated for western blot detection across human, mouse, and rat samples, supporting comparative studies of oxidative stress signaling, fibrotic remodeling, and vascular biology.

NOX4 is broadly expressed in renal tubular epithelium, vascular smooth muscle cells, cardiomyocytes, and lung parenchyma, and is widely investigated in models of diabetic nephropathy, cardiac hypertrophy, idiopathic pulmonary fibrosis, and tumor microenvironment biology, where its regulated ROS output influences TGF-beta signaling and extracellular matrix deposition. The reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, with a 100 µl volume that supports multiple blotting experiments. For best results, store at -20 degrees C and aliquot to limit freeze-thaw cycles across repeated uses. The unconjugated format permits flexible pairing with HRP, fluorophore, or biotinylated secondary reagents depending on the chosen experimental design, and cross-species reactivity enables consistent antibody usage between rodent disease models and human tissue samples within a single research program.