Anti-NOTCH1 Antibody

Supplied by Antibodies.com as 100 µl of liquid reagent in a single vial, this rabbit polyclonal antibody recognizes the human NOTCH1 receptor and is validated for western blot and immunohistochemistry workflows. NOTCH1 is a single-pass transmembrane receptor whose ligand-triggered proteolytic cleavage releases the Notch intracellular domain (NICD) to enter the nucleus and modulate transcription of HES and HEY family target genes, a signaling axis implicated in lymphocyte lineage commitment, vascular patterning, and a range of solid and hematopoietic cancers.

The reagent is unconjugated, allowing laboratories to pair it with their preferred HRP, biotin, or fluorophore-labeled secondary detection chemistry, and is intended for research use only. Refrigerated storage in the supplied vial is recommended for the working stock, and researchers preparing aliquots for repeated experiments should freeze them to limit freeze-thaw degradation of the antibody. As a polyclonal reagent, the antibody recognizes multiple epitopes across the immunogen, which can improve signal in denatured western blot applications compared with monoclonal alternatives.

Host species is rabbit, clonality is polyclonal, conjugate is unconjugated, and reactivity is reported for human samples; cross-reactivity with other species is not listed in the product specifications. Investigators studying NOTCH1 cleavage, NICD nuclear translocation, or downstream pathway activity in disease models commonly combine this reagent with appropriate positive and negative controls.