Anti-Notch 1 Antibody

This recombinant monoclonal reagent from Antibodies.com targets the transmembrane receptor Notch 1, a ligand-activated transcription factor central to the Notch signaling pathway that governs cell fate, differentiation, and proliferation across developmental and adult tissues. Supplied as 100 µl of unconjugated antibody in a single-use vial containing 1× TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, the reagent is validated for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and flow cytometry applications on human and mouse samples.

Notch 1 signaling controls lateral inhibition and binary cell fate decisions during embryogenesis, and aberrant pathway activity is linked to T-cell acute lymphoblastic leukemia, breast cancer progression, and vascular patterning defects. Researchers use this antibody to map receptor activation by detecting the transmembrane subunit or its cleaved intracellular domain (NICD) following ligand engagement. Storage at -20 °C in working aliquots is recommended to limit freeze-thaw cycles and preserve binding activity for repeated immunofluorescence and flow cytometry sessions.