Anti-NOS3 (phospho-T494) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes NOS3 (endothelial nitric oxide synthase, eNOS) only when phosphorylated at threonine 494, an inhibitory regulatory site targeted by protein kinase C signaling in vascular endothelium. Raised in rabbit and supplied as an unconjugated reagent at 1 mg/ml in phosphate buffered saline (pH ~7.2) with 0.05% sodium azide as preservative, the 100 µl format is intended for immunohistochemistry workflows on tissue sections derived from human, mouse, and rat samples. The phospho-specific reagent lets investigators distinguish the catalytically suppressed state of the enzyme from its basal active form, supporting studies of endothelial dysfunction, vascular tone regulation, shear-stress signaling, and pathways that modulate nitric oxide output in cardiovascular and metabolic disease research. Recommended use covers standard IHC protocols on fixed paraffin-embedded or frozen sections, with primary binding detected using anti-rabbit secondary reagents and chromogenic or fluorescence systems chosen by the end user. Because the reagent is phospho-selective, it complements total NOS3/eNOS antibodies and helps dissect dynamic regulation at this specific residue. Aliquot on receipt to limit freeze-thaw cycles; store refrigerated and protect from light. For laboratory research use only, not intended for diagnostic applications, clinical decision-making, or therapeutic use.