Anti-NOS3 (phospho-S1177) Antibody

This rabbit polyclonal antibody from Antibodies.com targets NOS3 (endothelial nitric oxide synthase) phosphorylated at Serine 1177, the activating modification catalyzed by Akt/PKB signaling. Raised in rabbit and supplied as an unconjugated reagent at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide (approx. pH 7.2), the antibody is validated for western blot and immunocytochemistry applications across human, mouse, and rat samples.

Phosphorylation at Ser1177 is a widely studied activation mark for eNOS, linking the enzyme's NO output to upstream kinase pathways including Akt and AMPK. Researchers use this reagent to monitor eNOS activation status in endothelial cell cultures, vascular tissue lysates, and cardiovascular disease models. The 100 µl vial format at 1 mg/ml concentration supports multiple western blot and immunocytochemistry experiments; store at -20°C in single-use aliquots to limit freeze-thaw cycles and protect from light.