Anti-NOS3 Antibody

NOS3, the endothelial isoform of nitric oxide synthase that converts L-arginine to nitric oxide in vascular endothelium, is widely studied in cardiovascular and endothelial biology. This rabbit polyclonal primary antibody from Antibodies.com is raised against NOS3 and supplied as an unconjugated reagent in a 100 µL vial, validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows.

The reagent cross-reacts with human, mouse, and rat eNOS, supporting experiments on cultured endothelial cells, aortic and cerebral tissue sections, and primary isolates from rodent disease models. Researchers commonly use the antibody to monitor eNOS expression under shear stress, hypoxia, or pharmacological challenge, where it serves as a marker of endothelial activation status. Working dilutions should be optimized empirically for each application, with the reagent detecting the canonical ~133 kDa eNOS band in Western blot preparations.

Short-term refrigerated storage is recommended, and the product should be aliquoted to minimize repeated freeze-thaw cycles. Researchers are advised to titrate the antibody against their specific sample type and confirm specificity using appropriate positive and negative controls.