Anti-NOS3 Antibody

This polyclonal antibody from Antibodies.com targets NOS3, the endothelial isoform of nitric oxide synthase responsible for producing nitric oxide in vascular endothelial cells. Raised in rabbit and supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the reagent is offered in a 100 µl vial volume and is validated for western blot and immunohistochemistry workflows. The antibody recognizes an epitope conserved across human, mouse, and rat NOS3, making it a practical tool for cardiovascular, vascular biology, and signal-transduction research programs that compare endothelial function across species. Endothelial NOS catalyzes the conversion of L-arginine to L-citrulline and NO, a reaction central to vasodilation, shear-stress signaling, and angiogenic pathways. Researchers studying atherosclerosis, hypertension, diabetes-related endothelial dysfunction, or ischemia-reperfusion injury frequently probe NOS3 expression and phosphorylation status (notably Ser1177 activation) using antibodies of this clonality. Storage recommendations for this azide-containing preparation include short-term refrigeration at 4°C and long-term aliquoting to limit freeze-thaw cycles of the stock solution. The unconjugated format leaves the user free to choose enzyme, fluorophore, or biotinylation chemistries during secondary detection, which is convenient when one antibody is reused across WB membranes, formalin-fixed paraffin-embedded sections, and fresh-frozen tissue in IHC.