Anti-NOS2 Antibody

This polyclonal rabbit antibody targets NOS2 (inducible nitric oxide synthase, iNOS), the calcium-independent isoform of the nitric oxide synthase family that catalyzes the production of nitric oxide from L-arginine during inflammatory responses. Raised in rabbit and purified as an IgG fraction, the reagent is unconjugated and supplied in a 100 µl vial, ready for use in western blot, immunohistochemistry, immunofluorescence, and flow cytometry workflows on human samples.

NOS2 is upregulated in macrophages, hepatocytes, and other cell types following stimulation with cytokines or bacterial lipopolysaccharide, and its expression is a well-characterized marker of M1 macrophage polarization and innate immune activation. The antibody is reactive with human NOS2 and is commonly cited in research on sepsis, neurodegeneration, atherosclerosis, and tumor microenvironment studies where nitrosative stress plays a mechanistic role.

As a primary antibody from Antibodies.com, this reagent supports standard detection protocols on cultured cells, formalin-fixed tissue sections, and isolated peripheral blood mononuclear cells. Aliquoting on receipt is recommended to limit repeated freeze-thaw cycles and preserve binding performance across multiple experiments.