Anti-NOS2 Antibody

Polyclonal rabbit antibody raised against inducible nitric oxide synthase (NOS2, also called iNOS), an approximately 130 kDa enzyme that catalyzes L-arginine to nitric oxide during inflammatory signaling. This unconjugated reagent is validated for Western blot and ELISA workflows and detects the endogenous protein in human, mouse, and rat samples, making it a practical choice for laboratories comparing inflammatory pathways across species in cell culture lysates and tissue extracts.

NOS2 is upregulated by cytokines and bacterial lipopolysaccharide in macrophages, microglia, and hepatocytes, and is widely studied in models of sepsis, neurodegeneration, ischemia-reperfusion injury, and tumor biology. Researchers use this antibody to track enzyme induction kinetics, differentiate M1-like macrophage polarization, and probe nitric oxide signaling in in vitro and in vivo experiments. The reagent is supplied by Antibodies.com as a 100 µL vial of polyclonal serum, unconjugated for flexible detection with secondary antibodies of the user's choice, including HRP, Alexa Fluor, or biotin conjugates for chemiluminescent, fluorescent, or affinity-based readouts.