Anti-NOS1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets neuronal nitric oxide synthase (NOS1), the constitutive calcium/calmodulin-dependent isoform that catalyzes the conversion of L-arginine to L-citrulline and nitric oxide in central and peripheral neurons. Raised in rabbits and supplied as an unconjugated IgG preparation in a 100 µl vial, the reagent is validated for western blot, immunohistochemistry, and immunoprecipitation workflows against human, mouse, and rat samples.

Researchers studying synaptic plasticity, neurovascular coupling, penile and gastrointestinal NANC neurotransmission, or skeletal muscle force production can use this polyclonal reagent to detect the ~161 kDa NOS1 band on western blots of brain, cerebellum, skeletal muscle, or epithelial lysates, or to localize NOS1-positive neurons and nerve fibers in fixed tissue sections by IHC. Immunoprecipitation of native NOS1 complexes from neural cell lysates is supported as well, making the antibody a flexible tool for expression profiling, isoform-distinction studies, and signaling-pathway characterization. Aliquot the vial upon arrival, store at -20 °C, and avoid repeated freeze-thaw cycles to preserve antibody activity across multiple experiments.