Anti-Nono Antibody

NONO (Non-POU Domain-Containing Octamer-Binding Protein), historically known as NonO or p54nrb, is a multifunctional nuclear factor implicated in RNA splicing, transcriptional regulation, DNA double-strand break repair, and circadian clock gene expression. This rabbit polyclonal reagent from Antibodies.com was raised against NONO and is supplied as 100 µl of unconjugated antiserum in a single-use vial. The antibody binds human, mouse, and rat NONO and supports western blot analysis of nuclear extracts, immunohistochemistry on paraffin-embedded tissue sections, and immunofluorescence detection in cultured cells. Polyclonal recognition of multiple epitopes improves tolerance for post-translational modifications and minor conformational changes introduced by fixation. The unconjugated presentation leaves the choice of reporter open: enzyme, biotin, or fluorescent secondary reagents can be matched to the chosen application without prior chemical modification of the primary. Researchers investigating X-linked intellectual disability associated with Xq13.1 microdeletions, paraspeckle assembly dynamics in partnership with SFPQ, or epithelial-mesenchymal transition signaling in prostate cancer models rely on this reagent to map NONO subcellular localization and modification states. Aliquot upon receipt and store at 4 °C for short-term use, or at -20 °C in working aliquots to limit freeze-thaw cycles over multi-week experiments.