Anti-NOLC1 Antibody

A rabbit polyclonal antibody raised against NOLC1, supplied unconjugated as an IgG reagent by Antibodies.com and purified by affinity chromatography. It is listed as reactive with human samples in the supplier's catalogue entry, with the manufacturer's product page additionally indicating cross-reactivity with mouse and rat samples (C0; S1).

According to the manufacturer, the antibody is validated for Western blot (WB), immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence (ICC/IF). Recommended dilutions are 1:1,000–1:2,000 for WB, 1:50–1:200 for IHC, and 1:50–1:200 for ICC/IF, with the target band reported at a molecular weight of 120 kDa (S1).

The immunogen is a recombinant fusion protein corresponding to amino acids 400–699 of human NOLC1 (NP_004732.2). The product is supplied as a liquid in Phosphate Buffered Saline, pH 7.3, containing 50% glycerol and 0.02% sodium azide. It is shipped at 4 °C; upon delivery it should be aliquoted and stored at –20 °C, with freeze/thaw cycles avoided (S1).