Anti-NMNAT1 Antibody

Raised in rabbit against NMNAT1 (nicotinamide mononucleotide adenylyltransferase 1), this polyclonal primary antibody from Antibodies.com recognizes the human form of the enzyme that catalyzes the final step of nuclear NAD+ biosynthesis, converting NMN and ATP into NAD+. The unconjugated reagent is supplied as 100 µl of a 1 mg/ml solution in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant.

Recommended applications include western blot for detecting endogenous NMNAT1 expression in cell lysates and immunohistochemistry for localizing the protein in fixed tissue sections. Because the antibody reacts with human NMNAT1, it supports studies of NAD+ metabolism in cell lines and primary cells of human origin, as well as investigations into Leber congenital amaurosis type 9 (LCA9), a recessive retinal dystrophy linked to loss-of-function mutations in the NMNAT1 gene.

Aliquot upon receipt and store the glycerol-containing stock at -20°C; for short-term use, keep refrigerated. Avoid repeated freeze-thaw cycles to preserve binding activity. The vial format delivers enough reagent for multiple western blots and staining runs depending on dilution.