Anti-NLE1 Antibody

NLE1 (Notchless homolog 1) is a WD40-repeat-containing protein associated with Notch signaling and 60S ribosomal subunit biogenesis in mammalian cells, contributing to pre-rRNA processing during ribosome assembly. This rabbit polyclonal antibody from Antibodies.com was raised against human NLE1 to support studies of ribosomal assembly, Notch pathway regulation, and translation-related cell biology in human systems.

The reagent is supplied as a 50 µL liquid aliquot of unconjugated IgG, validated for Western blot (WB) applications on human samples. Each preparation is affinity purified and quality-tested on relevant cell lysates, with reactivity confirmed for human NLE1. For detection, pair with an HRP-conjugated anti-rabbit secondary antibody and a chemiluminescent substrate on standard PVDF or nitrocellulose membranes, optimizing dilution empirically for each lysate and transfer condition.

Aliquot upon first use and store at -20 °C; avoid repeated freeze-thaw cycles by freezing in working volumes and minimizing light exposure of the unconjugated stock. For research use only; not intended for diagnostic or therapeutic applications.