Anti-NLE1 Antibody

Supporting research into eukaryotic ribosome assembly, this unconjugated primary antibody reagent from Antibodies.com targets human NLE1 (Notchless protein homolog 1), a conserved WD40-repeat assembly factor of the pre-60S Rix1 complex that drives 60S ribosomal subunit maturation through its MIDAS–Ubl interaction with the AAA+ ATPase MDN1/Rea1. The rabbit polyclonal IgG reagent is affinity-purified and supplied as a 100 µl liquid aliquot without fluorescent or enzymatic tags, allowing direct integration into chemiluminescent western blot pipelines that resolve endogenous NLE1 expression at its predicted 53 kDa band. Verified on human lysates under standard denaturing SDS-PAGE conditions, it supports investigations into Notch signalling turnover, p53-mediated ribosomal stress responses in hematopoietic stem cells, and the elevated NLE1 expression reported in melanoma and breast cancer contexts. Researchers preparing whole-cell extracts from HeLa, HEK293, or Jurkat lines can dilute the antibody within standard IgG working ranges, with brief centrifugation before first use and refrigerated storage between sessions to preserve activity across multiple freeze-thaw cycles. The polyclonal format exposes the reagent to multiple epitopes along the target, a useful property when probing low-abundance isoforms or partially processed assembly intermediates captured in pre-60S particles.