Anti-NKX2-5 Antibody

This Rabbit polyclonal reagent from Antibodies.com is raised against NKX2-5, a homeobox transcription factor also known as CSX, Cardiac-specific homeobox, Homeobox protein Nkx-2.5, or NKX2E. The immunogen is a recombinant human NKX2-5 protein, and the antibody has been affinity-purified using that immunogen so it specifically recognizes the endogenous level of total NKX2-5 protein. Supplied as an unconjugated polyclonal antiserum at 1.0 mg/mL in a 50 µl aliquot, it is formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The host species is rabbit and the format is polyclonal, validated for western blotting (WB) and immunohistochemistry (IHC) on human, mouse, and rat samples, making it suitable as a cardiomyocyte marker in cardiac developmental and stem-cell studies. The product is shipped at 4 °C for stability; upon receipt it should be aliquoted and stored long-term at −20 °C, avoiding repeated freeze/thaw cycles. For research use only, this affinity-purified primary antibody offers a reliable tool for probing NKX2-5 expression across multiple species and tissue types.