Anti-NKX2-5 Antibody

Raised in rabbit against NKX2-5, the homeodomain transcription factor also called CSX that drives cardiac progenitor specification, ventricular chamber formation, and maintenance of the atrioventricular conduction system. Produced by Antibodies.com as an unconjugated polyclonal IgG in 100 µL aliquots, this reagent is validated for Western blot and immunohistochemistry on Human, Mouse, and Rat samples. NKX2-5 localizes to cardiomyocyte nuclei from early embryonic stages through adulthood, and loss-of-function studies in vertebrate models link reduced activity to atrial septal defects, ventricular septal defects, and progressive atrioventricular conduction block, which is why cardiovascular and developmental biology groups apply this antibody to track differentiation of iPSC-derived cardiomyocytes, profile congenital heart disease material, and map conduction-system lineage. Storage at -20 °C in working aliquots preserves activity through multiple freeze-thaw cycles; for short-term bench use keep refrigerated and protect from light to maintain titer.