Anti-NHEJ1 Antibody

NHEJ1 (also called XLF or Cernunnos) operates as a scaffolding factor in the classical non-homologous end joining pathway, partnering with XRCC4, Ku70/80, and DNA-PKcs to ligate DNA double-strand breaks generated during V(D)J recombination, class switch recombination, and genotoxic stress. Loss-of-function mutations in NHEJ1 are linked to radiosensitive severe combined immunodeficiency with microcephaly, underscoring its role in lymphoid and neural development.

This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against human NHEJ1 and validated for Western blot, immunohistochemistry, and immunofluorescence in human, mouse, and rat samples. The 50 µl vial format supplies the primary antibody at working concentration without fluorophore or enzyme conjugation, allowing researchers to pair it with their preferred secondary detection chemistry for fluorescence imaging, Western blot detection, and immunohistochemistry of fixed tissue sections.

Recommended applications include monitoring endogenous NHEJ1 expression across lymphoid cell lines, examining DNA repair foci following irradiation, and characterizing patient-derived lymphoblastoid lines carrying NHEJ1 variants. Refrigerate upon receipt; protect from light and avoid repeated freeze-thaw cycles to preserve activity. For research use only.