Anti-NHEJ1 Antibody

NHEJ1, also called XLF or Cernunnos, is a core component of the classical non-homologous end joining (NHEJ) pathway, the dominant mechanism for repairing DNA double-strand breaks in mammalian cells. This rabbit polyclonal antibody from Antibodies.com was raised against the NHEJ1 protein and is unconjugated, leaving the choice of secondary reagent and detection chemistry to the user. It has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows, and it cross-reacts with human, mouse, and rat samples, enabling comparative work across standard laboratory models.

The reagent is supplied as a 100 µl liquid aliquot of IgG and is suited for probing endogenous NHEJ1 expression in whole-cell lysates, formalin-fixed tissue sections, and cultured cells. Investigators studying V(D)J recombination, ionizing-radiation sensitivity, microcephaly, or radiosensitive severe combined immunodeficiency can use this antibody to track NHEJ1 localization at damage foci and to monitor changes in protein abundance after genotoxic treatment. Typical experimental uses include co-immunoprecipitation with XRCC4 and DNA Ligase IV to map NHEJ complex assembly, immunofluorescence imaging of irradiation-induced nuclear foci, and immunoblotting of patient-derived lymphoblastoid lines to assess NHEJ1 status.

For handling, store refrigerated, protect from light, and aliquot to limit freeze-thaw cycles if the antibody will be used over an extended period. This polyclonal preparation is supplied for research use only and is not intended for clinical or diagnostic purposes.