Anti-NGFRAP1 Antibody

A rabbit polyclonal antibody raised against NGFRAP1, offered by Antibodies.com as a primary antibody reagent for research use. The antibody is unconjugated and of the IgG isotype, produced in rabbit and purified by affinity chromatography. It is reactive with human and mouse samples.

Validated for Western blot and immunocytochemistry/immunofluorescence applications, with recommended dilutions of 1:500–1:2,000 for WB and 1:50–1:100 for ICC/IF. The immunogen is a recombinant fusion protein corresponding to amino acids 1–111 of human NGFRAP1 (NP_996798.1). The target protein has a molecular weight of 24 kDa. Supporting Western blot data were generated using lysates loaded at 25 µg per lane, with a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at 1:10,000 dilution, 3% non-fat dry milk in TBST as blocking buffer, ECL Enhanced Kit (RM00021) detection, and a 90 s exposure. Immunofluorescence was performed on MCF-7 cells.

The product is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. Concentration is lot specific. It is shipped at 4°C; upon receipt, aliquot and store at −20°C, avoiding repeated freeze/thaw cycles.