Anti-NGF Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against nerve growth factor (NGF) using a synthetic peptide of human NGF as the immunogen, and the resulting IgG fraction is affinity-purified on the immunogen to ensure selective recognition of endogenous total NGF protein. Supplied at 1.0 mg/mL in a 50 µl volume of phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the unconjugated antibody ships at 4 °C and should be stored at –20 °C, with freeze/thaw cycles avoided to preserve activity. Validated applications cover Western blotting (WB) and immunohistochemistry (IHC), and the antibody has been confirmed to react with human, mouse and rat samples, making it suitable for cross-species studies of neurotrophin signalling in neural, inflammatory and peripheral tissue contexts. The polyclonal host response provides broader epitope coverage than a monoclonal, which is particularly helpful when probing the pro- and mature forms of NGF that arise from proteolytic processing of the precursor. Intended for research use only, this primary antibody offers a reliable tool for laboratories characterising NGF expression in cell lysates, tissue sections and disease models, with consistent batch-to-batch performance backed by the supplier's quality control standards.