Anti-NGB Antibody

From Antibodies.com, this is a rabbit polyclonal antibody raised against Neuroglobin (NGB), supplied as an unconjugated IgG reagent in liquid form at a lot-specific concentration. The catalogue listing reports reactivity with human, mouse and rat samples and applications in Western blot and immunofluorescence, while the manufacturer specifications for this entry indicate reactivity with mouse samples and validated applications in Western blot and ELISA.

The immunogen is a synthetic peptide corresponding to amino acids 1–151 of human Neuroglobin, with the antigen species given as human. The antibody is affinity-purified and formulated in phosphate-buffered saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. The predicted molecular weight of the target is 17 kDa. Recommended dilutions are 1:500–1:1,000 for Western blot, with a starting concentration of 1 µg/ml for ELISA. The reagent is shipped at 4°C; on receipt it should be aliquoted and stored at –20°C, avoiding repeated freeze/thaw cycles.