Anti-NFS1 Antibody

A polyclonal antibody targeting NFS1 cysteine desulfurase, the mitochondrial enzyme responsible for mobilizing sulfur during the de novo assembly of iron-sulfur (Fe-S) clusters. This unconjugated reagent from Antibodies.com is raised in rabbit and supplied as 100 µl of ready-to-use antibody, validated for western blot (WB) and immunofluorescence (IF) workflows with confirmed cross-reactivity across human, mouse, and rat NFS1 orthologs.

NFS1 functions as the primary sulfur donor for Fe-S cluster biogenesis, feeding sulfur through the ISCU scaffold to build Fe-S cofactors that underpin mitochondrial respiration, DNA repair, and metabolic regulation. Researchers investigating mitochondrial iron handling, Friedreich's ataxia models, or cancers with altered iron-sulfur metabolism (such as pancreatic ductal adenocarcinoma and certain leukemias) routinely use anti-NFS1 tools to track expression changes, monitor mitochondrial stress responses, and dissect how cluster assembly reshapes under pathological conditions.

The antibody is unconjugated, allowing flexible pairing with secondary reporters of choice (HRP, fluorophores, or biotin) depending on assay design. Its polyclonal nature provides epitope diversity useful for recognizing NFS1 even when post-translational modifications or species-specific sequence variation are present. Recommended handling follows standard cold-chain practice: refrigerate the vial in single-use aliquots to preserve activity across repeated freeze-thaw cycles, and protect from light when working with fluorescent secondary detection.

Each vial is shipped from Antibodies.com with full reactivity documentation covering the three validated species and application notes supporting both WB band detection and IF subcellular localization of the mitochondrial NFS1 signal.