Anti-NFKBIL1 Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes NFKBIL1 (NF-kappa B inhibitor-like 1), a cytoplasmic modulator encoded within the MHC class III locus on human chromosome 6p21.3. The reagent is supplied as an unconjugated IgG preparation in a 200 µl vial, intended for western blot detection of endogenous human NFKBIL1 in cell and tissue lysates.

NFKBIL1 participates in negative feedback regulation of the NF-κB transcription factor complex, influencing TNF-alpha-driven inflammatory cascades and adaptive immune responses. Investigators probing autoimmune-associated loci or NF-κB pathway cross-talk use this reagent in immunoblotting workflows, often pairing it with anti-rabbit HRP secondary antibodies for chemiluminescent readout.

For storage, aliquot the antibody upon receipt and freeze at -20°C; working stocks containing 50% glycerol can remain stable through repeated freeze-thaw cycles. Each lot is validated against the recombinant human protein to confirm species specificity and minimal off-target binding in HeLa and HEK293 lysates.