Anti-NFKBIE Antibody

NFKBIE encodes IκBε, a cytoplasmic member of the inhibitor of NF-κB (IκB) family that sequesters NF-κB transcription factor dimers in resting cells and releases them upon stimulus-induced phosphorylation and proteasomal degradation. This rabbit polyclonal IgG reagent from Antibodies.com is raised against the IκBε protein and is validated for western blot detection of endogenous IκBε levels in mouse samples, supporting studies of both canonical and non-canonical NF-κB pathway activation in immune cell populations.

The antibody is supplied as 100 µl of unconjugated liquid, allowing laboratories to pair it with their preferred secondary detection chemistry, including HRP-based chemiluminescence or fluorescence imaging workflows. Its polyclonal profile broadens epitope coverage across the target, which is useful when tracking stimulus-induced changes in IκBε abundance, phosphorylation status, and turnover kinetics downstream of B-cell receptor, T-cell receptor, and Toll-like receptor engagement.

Common research applications include probing T- and B-cell receptor signaling cascades, profiling inflammatory cytokine responses, and comparing wild-type versus NFKBIE knockout phenotypes in mouse models of immune dysregulation. The reagent is also relevant to investigations of recurrent NFKBIE mutations that have been catalogued in activated B-cell-like diffuse large B-cell lymphoma (ABC-DLBCL) and related lymphoid malignancies, where altered IκBε function is implicated in constitutive NF-κB activation and tumor cell survival.