Anti-NFKBIA Antibody

Raised in rabbit and affinity-purified against a recombinant human NFKBIA immunogen, this unconjugated polyclonal antibody from Antibodies.com is supplied at 1.0 mg/mL in a 50 µL aliquot. The reagent is validated for Western blot, immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and immunoprecipitation (IP), and it cross-reacts with Human, Mouse, and Rat samples, detecting the endogenous level of total NFKBIA protein. Its formulation is phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. Antibodies.com ships the vial at 4°C; upon receipt it should be aliquoted and stored at –20°C, with repeated freeze/thaw cycles avoided to preserve activity. Representative validation data published on the supplier page include a Western blot on HL60 cell lysate, IHC on paraffin-embedded human kidney and rat testis (both at 1:200), and immunofluorescence on HeLa cells with DAPI nuclear counterstaining. The target is also referenced by the synonyms I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, MAD3, and NF-kappa-B inhibitor alpha, and the product is supplied for research use only.