Anti-NFKBIA Antibody

The NFKBIA protein, also known as IκBα, functions as the principal cytoplasmic inhibitor of the NF-κB transcription factor family. In resting cells, NFKBIA binds NF-κB dimers and masks their nuclear localization signals, retaining the complex in the cytosol until pathway activation.

Upon stimulation by cytokines such as TNFα, pathogen-associated molecules, or cellular stress, the IKK complex phosphorylates NFKBIA on serine residues, triggering its ubiquitination and proteasomal degradation, which liberates NF-κB to drive transcription of inflammatory and survival genes. This rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of unconjugated antiserum and is validated for Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows using human, mouse, and rat samples.

Researchers use this Anti-NFKBIA Antibody to monitor canonical NF-κB signaling dynamics across multiple readouts. Western blot experiments reveal the characteristic stimulus-induced disappearance of the IκBα band within minutes of TNFα or LPS challenge, providing an indicator of pathway engagement. In immunofluorescence and immunohistochemistry, the antibody highlights cytoplasmic NFKBIA distribution and reveals redistribution during signaling, including nuclear translocation of partner NF-κB subunits.

The polyclonal format is well suited for immunoprecipitation studies aimed at dissecting NFKBIA interactomes, capturing endogenous complexes with p65/RelA, p50, and other pathway components. Applications extend to investigations of immune regulation, inflammatory disease biology, tumor microenvironment signaling, and genetic disorders caused by NFKBIA mutations such as anhidrotic ectodermal dysplasia with immunodeficiency. For best results, aliquot upon receipt to limit freeze-thaw cycles and protect from light during extended incubations.