Anti-NFKB1 Antibody

The NFKB1 gene encodes the p105 precursor protein, which is processed into the p50 subunit of the NF-kB transcription factor complex. This primary reagent from Antibodies.com is a rabbit polyclonal reagent raised against NFKB1 and supplied at 100 uL per vial in unconjugated form, allowing researchers to pair it with their own choice of secondary labels for downstream detection.

Validated for Western blot and immunofluorescence workflows, the polyclonal reagent detects NFKB1 in human, mouse, and rat samples. Its polyclonal profile increases the likelihood of recognizing the target across multiple epitopes, which is helpful when probing both the p105 precursor and the cleaved p50 subunit generated through proteasomal processing.

Researchers studying inflammation, immune signaling, stress responses, apoptosis, and oncogenic pathways commonly use this reagent. Because NF-kB activity is regulated by IkB sequestration and IKK-mediated phosphorylation, investigators often combine western blot detection of NFKB1 with phospho-specific antibodies or cytoplasmic versus nuclear fractionation to track pathway activation.

The unconjugated format makes the reagent compatible with HRP, fluorophore, or biotin-based detection chemistries, and it can be applied to fixed cell preparations for immunofluorescence imaging of NFKB1 localization. Store refrigerated and avoid repeated freeze-thaw cycles to preserve activity across multiple experiments.