Anti-NFkB-p65 (G530) Antibody

Produced in rabbit as an unconjugated polyclonal reagent, this Antibodies.com antibody recognizes the NFkB-p65 subunit, commonly referred to as RelA, with immunogen coverage directed at the region around residue G530. Supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the product is packaged as a 100 µl volume. The reagent shows reactivity with human, mouse, and rat samples and has been validated for western blot and immunohistochemistry workflows.

NFkB-p65 serves as the principal transactivating member of the NF-κB transcription factor family. The G530 residue sits within the linker region connecting the Rel homology domain to the C-terminal transactivation domain, a segment that influences IκBα interaction, nuclear translocation, and downstream transcriptional output following canonical pathway activation. Investigators working in inflammation biology, immune signaling, cancer research, and cellular stress responses apply this reagent to monitor pathway engagement in cell culture lysates, freshly prepared tissue homogenates, and formalin-fixed paraffin-embedded sections.