Anti-NFkB-p65 (Ab-529) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against the NFkB-p65 subunit (also known as RelA), a central transcription factor of the canonical NFkB signaling pathway that drives inflammatory, immune, and stress-response gene expression in human cells. The reagent is supplied as 50 µl of unconjugated antisera and is validated for Western blot and immunohistochemistry workflows, supporting detection of endogenous RelA levels in lysates and fixed tissue sections from human samples. As a polyclonal reagent, it offers broader epitope coverage than monoclonal alternatives, which helps when probing denatured protein on membranes or formalin-fixed paraffin-embedded sections where accessibility to a single linear epitope can be limited. Researchers studying cytokine signaling, tumor necrosis factor pathways, oncogenic transformation, and immune cell activation routinely rely on p65 immunoblotting to monitor pathway engagement, while immunohistochemistry complements expression studies in translational research. The unconjugated format allows pairing with preferred secondary antibodies and detection chemistry, including HRP, biotin-streptavidin, or fluorescent systems, for flexibility across laboratory protocols. For storage, aliquot the antibody and store at -20 °C in working volumes to minimize freeze-thaw cycles and preserve activity across experiments.