Anti-NFkB-p65 (Ab-529) Antibody

Raised in rabbit against a synthetic peptide corresponding to residues 527-531 (L-L-S-G-D) of the human NFkB-p65 RELA transcription factor, this affinity-purified polyclonal reagent from Antibodies.com targets the p65 subunit (encoded by RELA, UniProt Q04206) that mediates canonical NF-kappaB signaling in response to inflammatory cytokines, pathogen-associated molecular patterns, and genotoxic stress. The unconjugated IgG is supplied at 1.0 mg/mL in phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.4, with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as cryoprotectant; the 100 uL vial format is shipped for short-term stability at 4 degrees C and should be aliquoted and stored frozen at -20 degrees C for longer projects, with repeat freeze-thaw cycles avoided. Recommended starting dilutions are 1:500 to 1:1000 for western blot detection of the ~65 kDa band in whole-cell lysates of TNF-alpha- or LPS-stimulated human tumor cell lines and 1:50 to 1:100 for immunohistochemistry on paraffin-fixed sections of inflamed or neoplastic human tissue. Because the reagent is unconjugated, it can be paired with anti-rabbit HRP, alkaline phosphatase, or fluorophore-conjugated secondaries of the investigator's choice to study constitutive versus inducible p65 nuclear translocation, IkappaBalpha degradation, and the consequences of small-molecule kinase or IKK inhibition in vitro in human cancer, autoimmune, and signal transduction research.