Anti-NFkB-p65 (Ab-468) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against the transcription factor NFkB-p65 (also known as RelA) when phosphorylated at serine 468, a modification associated with cytokine-driven inflammatory signaling and stress-activated kinase pathways such as those involving GSK-3β and CK2. The reagent is supplied as 100 µl of unconjugated antiserum and is validated for Western blot and immunohistochemistry workflows.

Reactivity spans human, mouse, and rat samples, which makes the antibody a practical option for translational studies bridging rodent disease models and human tissue specimens. In Western blot it is commonly applied to cell lysates after stimulation with TNF-α, IL-1β, or other NFkB-activating ligands, enabling researchers to follow Ser468 phosphorylation kinetics in parallel with total p65 levels. For immunohistochemistry, it supports localization of activated p65 in formalin-fixed, paraffin-embedded sections, which is useful for examining nuclear translocation in inflamed or neoplastic tissue.

Recommended handling practices include brief equilibration to room temperature before use, aliquoting to limit freeze-thaw cycles, and storing working aliquots under conditions that preserve phospho-specific binding. Pairing phospho-p65 detection with appropriate positive controls, such as TNF-α-stimulated HeLa lysates, and matched negative controls helps confirm signal specificity across applications.