Anti-NFkB-p65 (Ab-311) Antibody

Manufactured by Antibodies.com as a research-grade rabbit polyclonal reagent, this affinity-purified IgG preparation targets the transcription factor NFkB-p65 at a region centred on amino acids 309–313 (F-K-S-I-M). The unconjugated antibody is supplied at 1.0 mg/mL in a 50 µL aliquot of phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is shipped at 4 °C for short-term transit before being stored at –20 °C in single-use fractions to limit freeze/thaw damage. Endorsed for Western blotting (recommended dilution 1:500–1:1000) and immunohistochemistry (1:50–1:100), the reagent detects endogenous total NFkB-p65 across human, mouse, and rat samples, with a predicted molecular weight of approximately 65 kDa. Antibodies were produced by immunising rabbits with a synthetic peptide–KLH conjugate and subsequently purified by epitope-specific affinity chromatography, ensuring recognition of the native protein in lysate and tissue-section workflows.