Anti-NFkB-p65 (Ab-311) Antibody

The NFkB-p65 subunit (RelA) forms the canonical transactivation arm of the NFkB complex, regulating inflammatory and cell-survival gene programs in response to cytokines, pathogens, and cellular stress. From Antibodies.com, this polyclonal reagent was raised in rabbit against a synthetic peptide spanning the Ab-311 region of human NFkB-p65, providing a specific probe for this epitope in Western blot and immunohistochemistry workflows on human, mouse, and rat specimens.

Provided as 100 µl of unconjugated antiserum in a single vial, the antibody should be aliquoted into working volumes on arrival and stored at -20 °C, avoiding repeated freeze-thaw cycles to preserve binding activity. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, an antigen retrieval step is generally required to expose the target epitope, while Western blot applications benefit from comparing stimulated versus resting cell lysates to reveal the expected band differences. The polyclonal format offers multi-epitope recognition within the targeted region, which is useful when sample material is limited or when researchers are investigating subtle modification states around residue 311 of p65.