Anti-NFkB-p65 (Ab-276) Antibody

The NF-κB p65 (RelA) subunit sits at the core of canonical inflammatory and stress-response signaling, and this Antibodies.com rabbit polyclonal reagent was raised against an epitope mapping to the region surrounding amino acid 276 of human NFkB-p65. Supplied as an unconjugated IgG in a 100 µl vial, the antibody is ready to titrate into Western blot, immunohistochemistry, and immunofluorescence workflows.

Cross-reactivity is reported with human, mouse, and rat samples, allowing the same lot to be used across cell-line panels, primary cultures, and rodent tissue sections. On Western blot, the antibody supports detection of total p65 as well as stimulus-induced changes in pathway activation; on formalin-fixed paraffin-embedded sections, it enables chromogenic localization in tumor, inflammatory, and neural tissues; and on cultured cells, it supports immunofluorescence imaging of cytoplasmic versus nuclear p65 distribution following TNF-α, LPS, or other relevant stimulation.

Storage at -20 °C in small working aliquots is recommended to limit freeze-thaw damage to the polyclonal IgG, which carries multiple epitope specificities against p65 and can therefore accommodate modest variation in sample preparation. The unconjugated format lets researchers pair the reagent with HRP, biotin, Alexa Fluor, or other fluorophore-conjugated secondary antibodies depending on detection chemistry. Intended for research use only and not validated for diagnostic or therapeutic applications.