Anti-NFkB-p105 (V887) Antibody

Raised in rabbit against the NFkB-p105 precursor, this unconjugated polyclonal antibody from Antibodies.com detects the 105 kDa NFKB1 gene product that is processed to the p50 subunit of the NF-κB transcription factor family. The reagent is provided as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2.

Validated for western blot (WB) and immunohistochemistry (IHC) applications, the antibody shows reactivity with human, mouse, and rat samples, enabling consistent use across comparative models of inflammation, immune signaling, and cellular stress responses. It is well suited for tracking p105 abundance in cell lysates, monitoring stimulus-induced degradation of the precursor, or mapping its tissue distribution relative to processed p50 in fixed sections.

The polyclonal format offers multi-epitope coverage, which can be valuable when probing cleavage intermediates or post-translationally modified forms of the target. Suggested starting dilutions are 1:500 to 1:2000 for western blotting and 1:50 to 1:200 for immunohistochemistry, with further optimization per sample. The vial should be stored refrigerated; aliquot upon first use to limit freeze-thaw cycles and preserve antibody activity over time.