Anti-NFkB-p105 (phospho-S927) Antibody

This unconjugated polyclonal reagent targets the phosphorylated form of NFkB-p105 at serine 927, a regulatory mark on the IκB-like domain of the p105 precursor that has been associated with proteasomal processing to the p50 DNA-binding subunit of the NFkB transcription factor complex. By recognizing the modified epitope specifically, it allows researchers to distinguish signaling-active NFkB-p105 from total NFkB-p105 levels in cell lysates and tissue sections. Raised in rabbit, the reagent is intended for Western blot and immunohistochemistry workflows in studies of canonical NFkB pathway activation.

The antibody is supplied at 1 mg/ml in phosphate buffered saline (approx. pH 7.2) containing 0.05% sodium azide as a preservative. Each vial contains 100 µl of antibody solution, providing enough material for multiple membrane probes or staining experiments at standard working dilutions. The reagent from Antibodies.com shows reactivity with human, mouse, and rat samples, enabling cross-species comparisons of NFkB pathway engagement in inflammation, immune response, or cellular stress models.

For best results, store refrigerated at 4 degrees C to preserve phospho-specific binding activity, aliquot on first use to limit repeated freeze-thaw cycles, and protect from light during handling. As a research use only reagent within the primary antibody category, it is well suited to time-course and stimulation studies where p105 phosphorylation at S927 serves as a downstream readout of upstream kinase activity.