Anti-NFkB-p105 (Ab-932) Antibody

The NFkB signaling pathway sits at the core of inflammation, immune activation, and cellular stress responses, and the p105 subunit (encoded by NFKB1) acts both as a precursor to the p50 transcription factor and as an IκB-like inhibitor in resting cells. This rabbit polyclonal reagent from Antibodies.com binds an epitope centered on residue 932 of human NFkB-p105, a region that overlaps the IKK-dependent phospho-acceptor sites used to trigger proteasomal processing of p105 to p50. The antibody is supplied as 100 µl of unconjugated antiserum for Western blot and immunohistochemistry workflows, and the reported reactivity covers endogenous protein in human, mouse, and rat samples.

For Western blot, the reagent is used on whole-cell lysates prepared under denaturing conditions, with detection of the full-length ~105 kDa precursor band and, in stimulated samples, the processed ~50 kDa fragment. In IHC, the polyclonal format gives broader epitope coverage on formalin-fixed, paraffin-embedded sections, useful when mapping p105 distribution across inflamed or tumor tissue. Because the antibody is unconjugated, it pairs with HRP- or fluorophore-labeled secondary reagents of the researcher's choice. Storage is at -20 °C in working aliquots to limit freeze-thaw cycles, with refrigeration acceptable for actively used vials. For research use only.