Anti-NFkB-p100 (S865) Antibody

Raised in rabbit against the phosphorylated Ser865 epitope of the NFkB-p100 precursor, this polyclonal reagent from Antibodies.com is supplied as 100 µl of liquid antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. The unconjugated antibody is validated for western blot, immunohistochemistry, and immunoprecipitation workflows, with confirmed reactivity against human, mouse, and rat samples, supporting pathway studies of non-canonical NF-κB signaling where stimulus-driven processing of p100 to p52 is a key regulatory event. Applications include monitoring site-specific phosphorylation at Ser865 in cell lysates, immunoprecipitating endogenous NFkB-p100-containing complexes, and localizing the precursor in fixed tissue sections. Handling notes: thaw the vial on ice before use, mix gently without vortexing, and if extended storage beyond the working day is required, hold refrigerated and aliquot to limit freeze-thaw cycles.