Anti-NFE2L2 Antibody

NFE2L2 (also known as Nrf2, Nuclear factor erythroid 2-related factor 2) is a basic leucine zipper transcription factor that acts as the master regulator of the cellular antioxidant response, driving expression of phase II detoxification and cytoprotective genes through the antioxidant response element (ARE). This unconjugated rabbit polyclonal primary antibody from Antibodies.com targets NFE2L2 and is supplied as 100 µL of antiserum.

Validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), the reagent reacts with human, mouse, and rat samples. Researchers deploy it to track endogenous NFE2L2 abundance, Keap1-mediated stabilization, and nuclear translocation under oxidative stress, electrophilic insult, or pharmacological Nrf2 activation.

For western blotting, lysates are resolved by SDS-PAGE and probed with HRP- or fluorescently-labeled secondary antibodies; in IHC and IF, the unconjugated format supports flexible pairing with species-matched secondaries and tyramide- or polymer-based amplification. Recommended handling: store at -20 °C in working aliquots to limit freeze-thaw cycles and protect from light during longer-term storage.