Anti-NFATC1 Antibody

The NFATC1 target (Nuclear Factor of Activated T Cells, Cytoplasmic 1) is a calcium-regulated transcription factor best known for linking calcineurin signaling to inducible gene programs in immune and developmental contexts. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against NFATC1 and validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human and rat samples.

The antibody is supplied as a 100 µl volume at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, ready to dilute into standard blocking and incubation buffers. Because NFATC1 translocates from the cytoplasm into the nucleus after calcineurin-mediated dephosphorylation, immunofluorescence can be used to monitor subcellular redistribution in stimulated T cells and other responsive cell types, while Western blot and IHC support detection of total protein across tissues and lysates. The polyclonal format gives broader epitope coverage than a typical monoclonal, which is useful when probing the multiple NFATC1 isoforms or working with samples where the recognized epitope may vary between species.