Anti-NF2 (phospho S518) Antibody - Identical to Abcam (ab131473)

Phosphorylation of Neurofibromin 2 (Merlin) at serine 518 is a key regulatory switch that releases the tumor suppressor from the cortical cytoskeleton, and this rabbit polyclonal reagent from Antibodies.com is raised specifically against that phospho-epitope. Researchers use anti-phospho-S518 Merlin antibodies to monitor Merlin activation state in Neurofibromatosis type 2 studies, Schwann cell biology, meningioma-relevant pathways, and Hippo/YAP signaling experiments, since kinases such as PAK1/2 phosphorylate this residue to inactivate growth suppression.

Supplied as 100 µl of polyclonal serum in a single-use vial, the unconjugated IgG antibody is validated for Western blot (WB) and immunofluorescence (IF) workflows. Host species is rabbit and reactivity has been confirmed against human, mouse, and rat samples, supporting cross-species studies in cell culture and primary tissue research. Working aliquots are best held at -20 °C to limit freeze-thaw cycles, with the vial protected from light in its original packaging when not in use.

Intended for research use only, the reagent pairs naturally with total Merlin or downstream pathway antibodies (YAP, TAZ, phospho-PAK) when comparing phospho-regulated versus total protein levels in normal versus transformed cells, and is commonly deployed to track contact-dependent growth inhibition and membrane-associated Merlin pools by immunostaining.