Anti-NFκB-p65 Antibody

NFκB-p65, also referred to as RelA, is the canonical transactivating subunit of the NF-κB transcription factor family and a central regulator of inflammatory signaling, innate immunity, and apoptosis. This polyclonal reagent from Antibodies.com is raised in rabbit and supplied at 100 µl per vial in an unconjugated liquid format, allowing straightforward integration into Western blot and ELISA workflows without the need for secondary labeling.

Validated uses include immunoblotting of endogenous p65 in whole-cell lysates and ELISA-based quantification, with reactivity confirmed against human, mouse, and rat samples. Aliquot the contents upon first thaw to limit freeze-thaw cycles and store at -20 °C in working volumes to preserve antibody performance through repeated experiments. Brief centrifugation before use helps recover material from the vial cap.

Investigators studying cytokine-driven pathways, tumor necrosis factor signaling, or stress-responsive gene regulation can use this reagent to monitor p65 activation, nuclear translocation, and IκBα-mediated turnover in primary cells, tissue extracts, and established cell lines. The unconjugated format also makes it compatible with custom HRP, biotin, or fluorophore conjugation when a defined secondary detection scheme is required.